Whenever I play my game I am creating, I get this message: "Please check your INTERNET EXPLORER settings

JavaScript is disabled in your Internet Explorer settings. Quest will not
work until you enable JavaScript".

As far as I know, JavaScript is enabled. Does Quest run on a Windows 7, 64 bit ultimate machine?

Check your IE settings - open Internet Explorer, go to Internet Options and then go to the Security tab.

The easiest thing to do would be to click the "Reset all zones to default level" button to reset each zone to its default settings. Otherwise, click Custom Level and scroll down to Scripting. Make sure that "Active Scripting" is enabled.

Yes, it is/was.

Is there any security software running that might be interfering with how applications are allowed to embed IE? It might be worth temporarily disabling it to see if that makes a difference.

I disabled my Anti-Virus & Anti-Adware program- no difference.

What result do you get if you load up this website in IE9? http://www.isjavascriptenabled.com/

It say's, "yes", in green on a grey background, to the question: " is javascript enabled".

Well I'm stumped then! May be worth checking what add-ons are running and disabling them. Failing that, a reinstall of IE9 may help.